Effects of Transcutaneous Application of Carbon Dioxide on diabetic muscle atrophy

Abstract

IntroductionDiabetes results in muscle atrophy, also called diabetic myopathy. Both the increase in muscle proteolysis and the decrease in muscle protein synthesis contribute to diabetic muscle atrophy. Transcutaneous application of carbon dioxide (CO2) has been used for cardiac disease and skin problems for a long time. In addition, it has been reported the CO2 treatment leads to an increase of muscle mass in normal rats. Therefore, the transcutaneous application of CO2 would have a therapeutic potential for diabetic muscle atrophy. The aim of the present study was to investigate the effects of transcutaneous application of CO2 on diabetic muscle atrophy.MethodsMale Goto‐kakizaki rats were divided into control and CO2 treatment groups. The hair on the lower limbs was shaved and the hydrogel was applied. The CO2 adaptor was attached to the limbs and sealed, and CO2 was administered into the adaptor for 30 min. This treatment was performed everyday for 8 weeks.ResultsThe body weight, epididymal and retroperitoneal fat were lower in the CO2 treatment group than in the control. In addition, the level of fasting blood glucose in the CO2 treatment group was significantly decreased compared with the control. Furthermore, the muscle masses of lower limbs in the CO2 treatment group were higher than those in the control.ConclutionThese results indicate that the transcutaneous CO2 treatment may have a therapeutic potential for diabetic muscle atrophy.Support or Funding InformationSupported by Grants‐in‐Aid for Science Research from the Japanese MEXT.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.