Abstract
When human plasma was mixed with 50 units of urokinase (UK) and clotted with thrombin in the presence of S-2251, the hydrolysis of S-2251 in the clot was higher than that in the plasma. The presence of 10 μM of tranexamic acid resulted in decrease in the activation rate of plasminogen in the clot but not in the plasma. Further increase in its concentration resulted in marked increase in plasminogen activation in both clot and plasma. The addition of 6-aminohexanoic acid or cis-AMCHA showed changes in the rate of plasminogen activation similar to that shown in the presence of tranexamic acid. Fifty μM of tranexamic acid totally inhibited urokinase induced clot lysis, while 1 mM of cis-AMCHA and 6-aminohexanoic acid totally inhibited clot lysis. The presence of 1 mM of thesewaminoacids remarkably increased the activation of plasminogen by UK, but no clot lysis was observed. Hydrolysis of 5-2251 was higher in the presence of tranexamic acid than in its absence or clot formation without tranexamic acid. Tranexamic acid seems to be more effective in enhancement of activation of plasminogen by UK than clot formation. Plasmin formed by UK was coexistent with α2PI. The presence of tranexamic acid or clot formation resulted in smaller extent of interaction of plasmin with α2PI, thus saving the exhaustion of α2PI and preventing instant inactivation of plasmin by α2PI.
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