Effects of traditional Chinese medicine on immune responses in abalone, Haliotis discus hannai Ino

Abstract

A traditional Chinese medicine (TCM) preparation was formulated from orange peel ( Pericarpium Citri Reticulatae), hawthorn ( Crataegus pinnatifida), astragalus ( Astragalus membranaceus ( Fisch.) Bunge), pilose asiabell root ( Radix codonopsis), indigowoad root ( Radix isatidis), taraxacum ( Herba taraxaci) and malt ( Fructus Hordei Germinatus) at a weight ratio of 1:1:1.5:1.5:1.5:1.5:2. A feeding experiment was conducted to determine the effects of TCM on innate immunity of abalone, Haliotis discus hannai Ino. Artificial diets containing 1%, 3%, 5% TCM preparation, 1% hawthorn or 1% astragalus, respectively, were fed to juvenile abalone (initial weight 10.38 ± 2.51 g; initial shell length 44.15 ± 4.15 mm) for 80 days. A TCM-free diet was used as a control. Each diet was fed to three replicate groups of abalone using a randomized design. The results indicated that phagocytic activity was significantly higher in abalone fed 3%, 5% TCM preparation, 1% astragalus or 1% hawthorn ( P < 0.05). Respiratory burst activity was significantly higher in abalone fed 1%, 3%, 5% TCM preparation, 1% astragalus or 1% hawthorn ( P < 0.05). Agglutination titre was significantly higher in abalone fed 5% TCM preparation ( P < 0.05). Weight gain ratio (WGR), daily increment in shell length (DISL), total haemocyte count (THC), plasma protein concentration, and the activity of acid phosphatase (ACP) were not significantly affected by the TCM preparation ( P > 0.05). These results indicate that TCM preparation can modulate the immunity of H. discus hannai, and it is very possible that TCM might be used as immunostimulants in abalone farming.