Abstract
Objective To study effects of titanium alloy (Ti) by composite treatment using sandblasting acid etehing ( SLA ) and alkali-heat ( AH ) on cyto-biocompatibility of MG63 cells,Methods Osteoblast-like MG63 cells were cultured at 2 × 104 or 1×105 cells per well on samples of group A ( pure Ti),group B (SLA Ti) and group C ( SLA-AH Ti).The outcomes of cell adherence rate,cell proliferation rate,alkaline phosphatase (ALP) special assay were evaluated and compared and cell morphology was observed by scanning election microscopy (SEM).Results In group A,group B and group C,the cell proliferation rate was 0.752 ±0.080,0.614 ±0.020 and 0.567 ±0.062,respectively ( P ＜0.05,group A vs group B or group C),the ALP special assay was 0.450 ±0.014,0.465 ±0.012 and 0.510 ±0.036,respectively ( P ＜0.05,group C vs group A).The cell adherence rate in group C showed higher than in the two other groups ( P ＜0.05 ),and the cells on composite treated Ti were attached well and spread more extensively.Conclusion The surface of composite treated Ti is biocompatible and better bioactive than that of pure Ti or SLA Ti. Key words: Titanium Alloy; MG63 cell; Biocompatibility
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