Effects of Tissue Factor and Tissue Factor Pathway Inhibitor-1 on Lung Adenocarcinoma With Venous Thromboembolism

Abstract

SESSION TITLE: Lung Cancer I SESSION TYPE: Original Investigation Poster PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM PURPOSE: To expore the relationship between lung adenocarcinoma and venous thromboembolic disease, several factors were dected, including coagulation indexes of PT, APTT, Fib, D-dimer and thromboembolic markers of TF, TFPI-1 in patients with lung adenocarcinoma; Meanwhile, to analyze the changs of TF / TFPI ratio in different stages of lung adenocarcinoma, so as to offer valuable evidence of whether those patients should receive anticoagulant therapy and how to choose the ideal anticoagulation time. METHODS: Fifty cases of lung adenocarcinoma patients, including 5 cases of venous thromboembolism were enrolled into the study from July 2013 to December 2014 in First Affiliated Hospital of Fujian Medical University. At the same time, 12 cases of patients with benign pulmonary disease, 20 cases of acute pulmonary thromboembolism and 30 cases of healthy people were also enrolled into the study. Expression of TF and TFPI-1 of lung tissue samples were detected by reverse transeriptase-polymerase chain reaction (RT-PCR) and SABC immunohistochemistry method. The content of plasma PT, APTT and Fib were detected by blood coagulation analyzer, the content of plasma TFAg and TFPI-1Ag were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: In thrombotic cases, PT, APTT and TFPI-1 decreased while Fib, D-dimer, TF and TF/TFPI ratio increased. PT in lung adenocarcinoma without VTE group showed no significant difference when compared with control group (P>0.05). APTT decreased while Fib and D-dimer values were remarkably elevated in lung adenocarcinoma group when compared with control group (P< 0.01). No matter in blood samples or tissue samples, we found that TF and TFPI-1 expressions significantly increased along with the advanced TNM stage of lung cancer. The plasma levels of TF and TFPI-1 in stagesⅠ, Ⅱof lung adenocarcinoma were higher than that in control group (P< 0.01) while the TF / TFPI ratio show no statistically significant difference between stages Ⅰ, Ⅱ and control group (P>0.05). CONCLUSIONS: PT, APTT, Fib and D-dimer could be used to preliminary evaluate the coagulation state of lung adenocarcinoma in clinical. TF/TFPI imbalance plays an important role on the mechanism of lung adenocarcinoma complicated with VTE. Other than the single index levels of TF or TFPI-1, the ratio of TF/TFPI can more accurately reflect the coagulation state in the patients with lung adenocarcinoma. CLINICAL IMPLICATIONS: TF/TFPI may play an important role on assessing whether or when the case with lung adenocarcinoma DISCLOSURE: The following authors have nothing to disclose: Chaosheng Deng, Xiaoming Cao, Qi-Chang Lin, Zhihua Huang, Haibo Ding, Jianchai Huang, Zhanghua Zhong, Yongxu Jin No Product/Research Disclosure Information