Abstract
Objective To study the effects of TRβΔ on apoptosis and proliferation of liver cancer cell line RH-35 from rat in vitro. Methods RH-35 cells were transfected by empty vector pcDNA3.1 and expression plasmid pcDNA3.1-TRβΔ, then exposure to 10 nmol/L T3. RH-35 cells apoptosis and proliferation were observed by flow cytometry and MTT colorimetric assay; Levels of catenin β-1(CTNNB1), senescence marker protein-30(SMP-30)and BCL2-antagonist/killer(BAK)mRNA evaluation were detected by quantitative real-time RT-PCR(RT-qPCR). Results In the presence of T3, overexpression of TRβΔ significantly inhibited the proliferation, increased the percentage of apoptotic, down-regulated CTNNB1and SMP-30 expression, up-regulated BAK expression in RH-35 cells(P<0.05). Conclusion TRβΔ could inhibit the proliferation of RH-35 cells and promote their apoptosis, which may be related to upregulation of BAK genes expression and downregulation of CTNNB1 and SMP-30 gene expression, and these effects could be regulated by T3. (Chin J Endocrinol Metab, 2016, 32: 691-695) Key words: Thyroid hormone receptor; TRβΔisoform; RH-35 cells
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