Effects of thermal stress on plasma concentrations of luteinizing hormone, progesterone, prolactin and testosterone in the cycling ewe

Abstract

Naturally cycling white faced ewes were utilized to study the effects of continuously elevated environmental temperature and/or humidity on plasma concentrations of luteinizing hormone (LH), prolactin (PRL), progesterone (P4) and testosterone (TE) during the estrous cycle. Fourteen ewes were randomly allocated on the day of estrus (day 0) to either thermoneutral conditions (21.1°C, 65% relative humidity) or elevated ambient temperature/humidity conditions (36.1°C, 71% relative humidity) producing an average 1.4°C hyperthermia. Animals remained in their respective environments and blood samples were collected daily until the next estrus or day 20, whichever occurred first. Starting at noon on day 14, blood was sampled every 2 hours. Concentrations of LH, PRL, P4 and TE were quantified using validated radioimmunoassays. Hyperthermic ewes exhibited 1) a significant decrease (P<0.05) in the incidence of behavioral estrus and a preovulatory LH surge at the expected time of the estrous cycle, 2) significantly lower (P<0.05) plasma P4 between days 7 and 13 of the cycle, 3) a six-fold increase of PRL levels (P<0.01). Plasma levels of TE were not significantly affected by hyperthermia. The only two experimental ewes which exhibited estrus and an LH surge also showed an unusual and significant peak in plasma P4 two days before estrus. These results confirm that elevated environmental temperatures that result in hyperthermia can induce endocrine imbalances in the ewe which may contribute to decreased reproductive efficiency in the heat-stressed female.