Effects of the synthetic estrogen 17-α-ethinylestradiol on Drosophila melanogaster: Dose and gender dependence

Abstract

17-a-ethinylestradiol (EE2) belongs to the increasing list of Endocrine Disruptors Chemicals (EDCs), able to interfere with the endocrine system in both vertebrates and invertebrates. Regardless of its great dispersion in the environment, to date there is still little knowledge about its action mechanisms and harmful effects in invertebrates. To better evaluate its potential role in invertebrates, we used the model system Drosophila melanogaster, an insect in which the hormonal response has been widely described.The effects of EE2 in D.melanogaster adults have been evaluated by using life traits as well as molecular endpoints. It was found that EE2 significantly decreases survival and fertility in both sexes, with a higher effect in female flies, as well as affects the expression of the Ecdysone Receptor (EcR), Estrogen Related Receptor (ERR), Yolk protein2 (Yp2) and yolkless (yl) genes. In conclusion, our results suggest that EE2 treatment may have potential toxic and endocrine effects on Drosophila melanogaster adults of both sexes. In particular, our data provide an indication that, after EE2 treatment, two of the genes involved in the vitellogenesis process (yl and Yp2) are transcribed in adult males where are mostly silent, and suggest future studies forward their use as potential molecular markers to EDCs exposure in Drosophila male.