Effects of the recombinant human growth hormone drug biosome on deep skin burn healing in rats

Abstract

The purpose of the study was to select the parameters of an experimental model of burn and to determine the effects of the recombinant human growth hormone drug biosome ("BIOTECHNA" CJSC, Lithuania) burn wound healing. Wistar rats were randomly divided into 2 groups: 1) those receiving placebo injections and 2) those given daily injections of Biosome in a dose of 2.0 mg/kg. A model of contact burn was used to reproduce a burn wound. The depth of the wound was determined, by morphologically assessing a burn wound tissue specimen on day О of burn application. The process of wound healing was associated with the change in the skin granulation defect area expressed as cm2. A statistically significant difference in the area of wounds in 2 animal groups was observed on healing day 10. On experimental day 22, the rat wound area in Group 2 decreased to an average of 0.26±O.I9 cm2, it was covered by crusts and regarded as healed. In Group I, the wound area achieved the similar one only on healing day 28. The difference in the duration of wound healing between the two groups was 9 days. The copper plate heated up to 240°C and pressed by a force of 1.56 n for 14 sec causes a deep rat skin bum of grade III B. The injections of Biosome reduced the healing of a deep skin burn in Group 2 rats by 27% as compared with Group I ones.