Abstract
Objective INF1 is a novel member of the formin family of cytoskeletal regulatory proteins. We previously identified a connection between INF1 expression and microtubule acetylation and more recently have found that INF1 expression induces Golgi dispersion. Given the dependence of ciliogenesis on microtubule acetylation and Golgi-dependent trafficking we wished to determine the effects of INF1 expression on cilia formation.
Highlights
We previously identified a connection between INF1 expression and microtubule acetylation and more recently have found that INF1 expression induces Golgi dispersion
Transient transfection was used to express a series of INF1 derivative in NIH 3T3 cells and the effects on cilia formation, cilia length and Golgi dispersion were monitored by immunofluorescence
INF1 expression inhibited ciliogenesis in the majority of NIH 3T3 fibroblasts, inhibition of ciliogenesis was not connected to INF1-induced Golgi dispersion
Summary
INF1 is a novel member of the formin family of cytoskeletal regulatory proteins. We previously identified a connection between INF1 expression and microtubule acetylation and more recently have found that INF1 expression induces Golgi dispersion. Given the dependence of ciliogenesis on microtubule acetylation and Golgi-dependent trafficking we wished to determine the effects of INF1 expression on cilia formation. Published: 13 July 2015 doi:10.1186/2046-2530-4-S1-P33 Cite this article as: Copeland et al.: Effects of the novel formin INF1 on ciliogenesis.
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