Abstract
Lanosol, found in the alga Odonthalia dentata, was administered to gumboot chitons ( Chryptochiton stelleri) by oral gavage at 0, 1, 2.5, 5, or 10 mg/kg for 4 days. C. stelleri feed on O. dentata, an alga avoided by most other herbivores. Digestive gland microsomes were probed for expression of various cytochrome P450s. CYP1A and CYP2-like protein expression was not detected in any individual ( n=22). Expression of a CYP3A-like protein was increased approximately 30% at higher doses. Estradiol hydroxylase activity increased with increasing dose, while testosterone hydroxylase activity declined with increasing dose. Anti-mammalian glutathione-S-transferase (GST) antibodies did not recognize any cytosolic chiton proteins. CDNB activity was highly variable and showed no relationship to lanosol dose. Lanosol non-competitively inhibited CDNB activity in digestive gland cytosol (IC 50=222 μM). This alteration of expression and activity of some Phase I and II biotransformation enzymes by lanosol may provide a selective advantage for chitons feeding on O. dentata.
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