Abstract
The effects of aqueous–organic solvent extraction and enzymic digestion sonication procedure on the isolation of spiked fluoroquinolones from poultry tissue have been studied. The highest recovery from spiked tissue was obtained using a mixture of trichloroacetic acid–acetonitrile (8:2) as extractant and an SDB1 cartridge for clean-up purposes. Validation data are presented for enrofloxacin, ciprofloxacin, sarafloxacin and difloxacin. The spiking procedure (spike contact time, spike solvent and matrix) had a small influence on the recovery of fluroquinolones from poultry muscle or liver. The effects of a different extraction on the determination of incurred enrofloxacin and its metabolite, ciprofloxacin, residues in poultry tissues have been investigated. The extraction procedures investigated – aqueous–organic solvent extraction, enzymic digestion or sonication – all gave similar results for incurred fluoroquinolone concentration in poultry muscle after correlation for spike recovery. The highest results were obtained in poultry liver when enzymic digestion has been used.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
