Effects of the in vitro treatment with gangliosides on the release of endogenous amino acids from rat hypoxic atria.

Abstract

1. In the rat isolated atria the in vitro exposure to 60 min of hypoxia in the absence of glucose followed by 30 min of reoxygenation increased the release of the amino acids glutamate (Glu) and taurine (Tau). The efflux of the remaining amino acids assayed (aspartate, glycine and alanine) did not change throughout the period studied. 2. The increase in Tau release started 45 min after the onset of the hypoxic period whereas that of Glu started during the reoxygenation phase. These increases were not observed when glucose was present during the hypoxic period. 3. The in vitro pretreatment for 2 h with 50 microM bovine brain gangliosides (BBG) prevented the increases in the release of Tau and Glu induced by the hypoxia reoxygenation. 4. These results constitute a further example where BBG appears to exert a protective role in cardiac tissues submitted to injuries.