Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation.

Seok Yong Kang,Jong-Seong Kang,Hyo Won Jung,Yong-Ki Park,Cheong-Weon Cho,Woo Kyung Kim,Young-Ho Kim,Hyo Sang Bae,Chong Woon Cho,Joo Hyun Nam

doi:10.1155/2017/8312946

Abstract

Ethnopharmacological Relevance In this study, we investigated the effects of Tribulus terrestris fruit (Leguminosae, Tribuli Fructus, TF) extract on oxazolone-induced atopic dermatitis in mice. Materials and Methods TF extract was prepared with 30% ethanol as solvent. The 1% TF extract with or without 0.1% HC was applied to the back skin daily for 24 days. Results 1% TF extract with 0.1% HC improved AD symptoms and reduced TEWL and symptom scores in AD mice. 1% TF extract with 0.1% HC inhibited skin inflammation through decrease in inflammatory cells infiltration as well as inhibition of Orai-1 expression in skin tissues. TF extract inhibited Orai-1 activity in Orai-1-STIM1 cooverexpressing HEK293T cells but increased TRPV3 activity in TRPV3-overexpressing HEK293T cells. TF extract decreased β-hexosaminidase release in RBL-2H3 cells. Conclusions The present study demonstrates that the topical application of TF extract improves skin inflammation in AD mice, and the mechanism for this effect appears to be related to the modulation of calcium channels and mast cell activation. This outcome suggests that the combination of TF and steroids could be a more effective and safe approach for AD treatment.

Highlights

Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease caused by abnormal skin barrier function and aberrant immune responses along with cutaneous hyperreactivity to environmental triggers [1]
To investigate the effects of TF extract on AD skin symptoms, symptom scores were measured in the dorsal skin of AD mice using a scoring index
An application of 1% TF extract with 0.1% HC on the dorsal skin of AD mice significantly (P < 0.001) reduced symptom scores compared to the control group (Figure 2(b))

Summary

Introduction

Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease caused by abnormal skin barrier function and aberrant immune responses along with cutaneous hyperreactivity to environmental triggers [1]. The main treatment for AD is skin hydration with emollients and suppression of cutaneous inflammation using topical steroids to reduce the number and severity of flares [1]. The use of steroids should be limited to the most severe cases due to their side effects, which include adrenal suppression, osteoporosis, hypertension, diabetes, obesity, and striae [3]. Evidence-Based Complementary and Alternative Medicine inhibitors, barrier repair therapies, and allergen-directed immunotherapy, have emerged. These therapeutic agents have greatly improved patient outcomes, the current treatments for AD are still not ideal and novel therapeutic strategies are required in the search for better drugs with safety and efficacy

Methods

Results

Discussion

Conclusion