Abstract

To investigate the cellular response to injury, analyzing histopathologic changes associated with increased cellularity, degeneration and disorganization of collagen fibers. Thirty wistar rats were divided in two groups after partial Achilles tenotomy: the right hind paw were treated with the essential oil of Alpinia zerumbet (EOAz), diluted to 33% (0.3 mL kg-1), and the left hind paw received sunflower oil for 3, 14, 30 and 90 days. Statistical significance was determined using a Chi-square and Pearson Correlation qualitative variables test. Moreover, Mann-Whitney U-test test for comparison between different groups of the same cell, one-way ANOVA, and Tukey's test of quantitative measurement. A decrease hyperemia (p < 0.001) was observed in the acute phase of inflammatory cell number (p < 0.001), whereas sub-acute phase was marked by significant correlation with macrophages in fibroblasts (r = 0.17, p = 0.03), with probable induction a dense and modeled tissue. At chronic phase, it was found an increase in the number of fibroblasts and a higher percentage of type I collagen fibers (78%) compared with control collagen fibers (55%). Oil of Alpinia zerumbet stimulated the process of maturation, organization and tissue repair which gave it greater resistance.

Highlights

  • Urban society seeks more means of physical or recreational activity to reduce stress and avoid physical inactivity

  • Samples from the group treated with essential oil of Alpinia zerumbet (EOAz) for 3 days after partial tenotomy of the Achilles tendon exhibited less significant hyperemia response than control group (Figure 5)

  • Investigate the degeneration and disorganization of collagen fibers, cellular response to injury, analyzing histopathologic changes associated with increased cellularity have been studied for atual researches

Read more

Summary

Introduction

Urban society seeks more means of physical or recreational activity to reduce stress and avoid physical inactivity. A significant increase in tendon injuries been has observed over the past 50 years. Achilles tendon injury have a high incidence and leads to functional limitations[2]. Experimental models are used to reproduce human pathologies and conduct studies, many resources are being using for the treatment of parcial tenotomy as example ultrasound[3]. After the injury the body initiates a process of tissue repair that can be divided into three phases: inflammation, proliferation and remodeling[1]. Inflammation histamine-induced [Ca2]i produces accumulation fibroblasts of human subcutaneous tissue, and is partially mediated by the release of ATP through the opening of Panx[1] hemi channels. Most studies have identified elevation of cytosolic Ca2 as an important regulator of ATP release in different cell models[4]

Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call