Abstract
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.
Highlights
Mango is the most delicious fruit with its health benefits and commercially valuable seasonal fruit
PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates
The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO47H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l
Summary
Mango is the most delicious fruit with its health benefits and commercially valuable seasonal fruit. Many mango fruit processing industries were established throughout the world and were generating huge amounts of waste or residues while processing the mango fruits. It has been reported that 28 to 43 % of the total of mangoes remain as residues mainly constituted by peel and seeds are discarded during the process (Guzman et al 2013). The mango industrial waste can be used in so many ways such as animal feed, fuel wood (dried seeds) and it can be useful for vermicompost preparation and biogas production. Mango industrial waste is the best source for the production of industrially important pectinase, as it contains high amounts of pectin in ripened mango (Yashoda et al 2005). Most of the Bacterial isolates such as Bacillus sp and Pseudomonas.sp., Bacillus sp
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