Effects of the C/N ratio and moisture content on the survival of ESBL-producing Escherichia coli during chicken manure composting

Abstract

Antibiotic-resistant bacteria are a threat to human and animal health. In recent years, the presence of extended-spectrum-β-lactamase (ESBL)-producing Escherichia (E.) coli in chicken manure, which is used as organic fertilizer, is a concern. The aim of the present study was to determine the effects of the carbon/nitrogen (C/N) ratio and moisture content (MC) on the survival of ESBL-producing E. coli during laboratory-scale composting of chicken manure. Nine different compost mixtures were enriched with an ESBL-producing E. coli strain to an initial concentration of 7 log10 CFU/g, and the number of E. coli, temperature, and chemical conditions during composting were determined. The fastest decrease in E. coli occurred for all mixtures with a C/N ratio of 10:1. Additionally, dry mixtures with an MC of 20% and a C/N ratio of either 10:1 or 40:1 exhibited faster reductions in E. coli than the moist mixtures did, despite having lower maximum temperatures within the bioreactors. The decimal reduction times ranged from 0.27 days in a mixture with a C/N ratio of 10:1 and 40% MC to 4.82 days in a mixture with a C/N ratio of 40:1 and 40% MC. Both the C/N ratio and MC had a significant effect on the number of ESBL-producing E. coli and on temperature development; the C/N ratio additionally affected the pH value and content of ammoniacal nitrogen during chicken manure composting. The results of this study demonstrate a considerable range of mechanisms involved in the inactivation of E. coli during chicken manure composting.