Abstract

Twelve lactating Holstein cows were placed on an experiment consisting of three periods: four-week normal control, six-week fat depression, and six-week treatment. In the last period the cows were divided into three groups and allotted to three treatments: I. Fat-depressing ration, II. Fat-depressing ration + 5% bentonite, and III. Fat-depressing ration + 10% bentonite.A highly significant increase in milk fat per cent was noted in both bentonite treatments versus the fat-depressing ration, but there was no significant difference between the two levels of bentonite. The cows in Treatments I, II, and III maintained per cent milk fat at 86, 144, and 144% of the fat-depressing period, and 60, 87, and 87% of the normal control period, respectively. Milk production was significantly higher for Treatment II. A significant increase in the proportion of rumen acetate, along with a decrease in propionate and valerate, was noted in the two bentonite treatments compared to the fat-depressing ration. There was a significant increase in the arteriovenous difference of blood acetate in the two bentonite treatments compared to the fat-depressing ration. Arteriovenous differences for blood glucose and ketones and plasma free fatty acids and triglycerides were not altered significantly.

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