Abstract
The effects of the β-isomer of hexachlorocyclohexane (β-HCH) on the induction of the cytosolic progesterone receptor (PgRc), on the redistribution of the estrogen receptor (ER), and its affinity for ER were investigated in the estrogen-sensitive human mammary tumor cell line MCF-7. The effects of β-HCH were compared to those of estradiol-17β (E 2) and 2,4,6-trichlorophenol (TCP), a major urinary metabolite of β-HCH in rats. β-HCH in concentrations higher than 1 μ m caused induction of PgRc whereas TCP was essentially without effect up to a cytotoxic concentration of 100 μ m. Furthermore, β-HCH (10 μ m) caused redistribution of ER, i.e., decrease of cytosolic ER concentration and increase of nuclear ER concentration, in a way similar to E 2. In contrast to this, β-HCH up to a molar excess of 6 × 10 4 (concentration 30 μ m) caused no significant displacement of [ 3H]E 2 from ER indicating that β-HCH has no substantial affinity for ER. It is concluded that β-HCH has estrogenic properties which are at variance with the failure to demonstrate binding of β-HCH to ER.
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