Abstract
This study investigated the effect of Teucrium polium L. essential oil (Tp EO) and α-pinene as its major component on the detoxifying- and intermediary-engaged enzymes of Ephestia kuehniella Zeller. Fourth instar larvae were separately fed on the treated artificial diets incorporated with LC50 concentration of Tp EO (4.91 μl ml-1) and α-pinene (10.66 μl ml-1). After three time intervals (24, 48, 72 hours), larval hemolymph was extracted to determine enzymatic activities. Activity of esterase, alanine and aspartate aminotransferases, aldolase, lactate dehydrogenase, and alkaline phosphatase were decreased in the treatments compared with the control. No significant differences were observed between some enzymes in the treated larvae highlighting their ineffectively role to detoxify and to metabolize Tp EO and α-pinene in the hemolyph of E. kuehniella larvae.
Highlights
One of the most effective ways to supply human food is proper storage of agricultural products in order to prevent them being wasted after harvest on farm
Survival, digestion and energy reserves of E. kuehniella larvae were determined after Teucrium polium L. essential oil (Tp EO) and α-pinene treatments (Shahriari et al, 2017b) while in the current study, we aimed to find the possible effects of Tp EO and α- pinene on detoxifying- and intermediary-engaged enzymes in E. kuehniella larvae
Our results demonstrated that Tp EO and α-pinene decreased all enzymes involved in intermediary metabolism of E. kuehniella, even phenoloxidase, which is a critical enzyme in immunity against entomopathogenic agents
Summary
One of the most effective ways to supply human food is proper storage of agricultural products in order to prevent them being wasted after harvest on farm. Control of stored product pests is commonly done by chemical gas pesticides, whose uncontrolled use of these compounds causes problems such as residues of toxins in food and the emergence of resistant populations (Negahban et al, 2007; Rajendran & Sriranjini, 2008). The existence of such problems has led researchers to seek alternative and less hazardous methods such as diatomaceous earth and powders of plants to efficient and safe control of stored product pest (Bohinc et al, 2013, 2018). These compounds disrupt intermediary metabolism and reduce the ability of the immune system against pathogenic agents (Zibaee & Bandani, 2010b; Shahriari et al, 2017b)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
