Effects of Tetracycline and Copper on Water Spinach Growth and Soil Bacterial Community

Abstract

The effects of tetracycline (TC) and copper (Cu) on the growth of water spinach and the bacterial community structure in soil were examined in this study. The results revealed that a single Cu treatment decreased water spinach development more severely than TC, and that the toxic effects of TC and Cu on water spinach were synergistic at low doses and antagonistic at high concentrations. The single Cu treatment had the largest influence on the activities of three antioxidant enzymes (Superoxide Dismutase (SOD), Peroxidase (POD), Catalase (CAT)) and the content of Malondialdehyde (MDA) in water spinach leaves, followed by the TC and Cu composed treatment, with the single TC treatment having the least effect. The results of 16Sr RNA sequence analysis showed that the richness and diversity of soil bacterial communities were reduced by either a single TC or Cu treatment. Cu had a greater effect on the composition of the microbial community at genus level than TC. In conclusion, Cu had a greater influence on the growth of water spinach and soil microbial community composition than TC. TC and Cu exhibited synergistic effects at low concentrations and antagonistic effects at high concentrations on relevant indicators when Cu concentration was fixed.