Abstract
BackgroundConsidering the prevalence of denture stomatitis and the challenge of controlling this pathology using conventional therapies, natural products have been suggested as important therapeutic alternatives due to their antifungal and anti-biofilm properties. Thus, this study investigated if immersion in Terminalia Catappa Linn. extract (TCE) affects Candida albicans biofilms developed on denture acrylic resin discs.Material and MethodsThe minimal inhibitory and minimal fungicidal concentrations (MIC and MFC, respectively) tests were performed for TCE against suspensions of C. albicans. For the biofilm assay, discs (10 x 2 mm) were fabricated using a denture acrylic resin with surface roughness standardized. The biofilms were allowed to develop for 24 hours. Then, they were immersed in the following treatments overnight (8 hours): phosphate-buffered saline (PBS, control), TCE at MIC, 5XMIC or 10XMIC. The biofilms were analyzed for cell counts and microscopy. Data were analyzed by ANOVA followed by a Tukey test at a 5% significance level.ResultsThe minimal concentration of TCE required to inhibit C. albicans was 6.25 mg/mL, while MFC was 12.5 mg/mL. Immersion in TCE at MIC was sufficient to reduce 80% of the biofilm viable cells compared to the control group (p< 0.001). Microscopic images confirm that immersion at 5XMIC and 10XMIC had a fungicidal activity with no significant differences between the concentrations regarding viable cells counts (p> 0.05).ConclusionsWithin the limitations of this study, it was possible to conclude that immersion in TCE reduced the C. albicans biofilms cells developed on the denture acrylic surface. Key words:Terminalia catappa Linn, Biofilm, Candida albicans.
Highlights
Candida albicans is the main microorganism involved in the pathogenesis of denture stomatitis (DS)
Given the lack of scientific information about T. catappa on C. albicans and the relevance of discovering new agents for the management of biofilm-dependent diseases, this study aims to investigate the effects of using T. catappa L. extract to reduce C. albicans biofilms that have formed on denture surfaces
Results -Susceptibility tests The analysis revealed that the amount of Terminalia Catappa Linn. extract (TCE) required to inhibit C. albicans planktonic cell growth ranged from 12.5 to 6.25 mg/mL, while the concentration required for fungicidal effects ranged from 25 to 12.5 mg/mL (i.e., MIC and MFC, respectively)
Summary
Candida albicans is the main microorganism involved in the pathogenesis of denture stomatitis (DS). The aim of this study is to evaluate if immersion in TCE has an effect on C. albicans biofilms developed on denture acrylic discs. The developed biofilms were immersed overnight (8 hours) at PBS (control), TCE at MIC, 5XMIC or 10XMIC. Results -Susceptibility tests The analysis revealed that the amount of TCE required to inhibit C. albicans planktonic cell growth ranged from 12.5 to 6.25 mg/mL, while the concentration required for fungicidal effects ranged from 25 to 12.5 mg/mL (i.e., MIC and MFC, respectively). Cells/mL measured the growth, and the results showed that exposure of TCE for 8 hours had a significant effect in comparison to the control group (p < 0.05), regardless of the concentration used. Microscope images confirmed that immersion at 5XMIC and 10XMIC have a fungicidal activity, with no significant differences among these concentrations regarding viable cells (p > 0.05)
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