Abstract

以从南亚热带水库中分离的拟柱孢藻(Cylindrospermopsis raciborskiiN8)为材料,研究了不同温度(12、16、20、24、28、32℃)、光照强度(6.6、12.4、21.5、30.7、62.9、106.4μmol/(m2·s))和硝态氮浓度(0.5、1、2、4、8、16、32、64、128mg/L)下拟柱孢藻的生长特性.结果表明:在实验设置的温度范围(16~32℃)内拟柱孢藻能够正常生长;最适温度范围为24—28℃,在28℃条件下,具有最大比生长速率,为0.189d^-1;当温度为12℃时,拟柱孢藻叶绿素a浓度一直降低,显著低于其他温度组(16~32℃).在6.6~106.4μmol/(m2·s)光照强度范围内,拟柱孢藻均呈指数增长趋势,最适光照强度为30.7μmol/(m2·s),其比生长速率达到最大值,为0.156d^-1;高光照强度(62.9~106.4μmol/(m2·s))下拟柱孢藻的比生长速率显著大于低光照强度(6.6—12.4μmol/(m2·s))处理组.拟柱孢藻开始指数增长的最低硝态氮浓度为4mg/L;硝态氮浓度为8mg/L时,拟柱孢藻达到最大比生长速率(O.155d。);当硝态氮浓度高于16ms/L时比生长速率增加不显著.高硝态氮浓度组(16~128ms/L)拟柱孢藻的叶绿素a浓度和比生长速率显著高于低硝态氮浓度组(0.5—2mg/L).研究结果说明拟柱孢藻对温度、光照和氮源均有较宽的生态位,有利于在较大空间尺度上进行扩散.

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