Abstract
1. 1. The connection between feeding regime (food deprivation and restricted diet) and thermal acclimation (1–2, 6, 11 and 16°C) was studied in rainbow trout held in diluted seawater (20‰S). 2. 2. At 1°C, food deprivation effects on all parameters are slight, and on RNA and certain enzymes they are masked by thermal acclimation effects. 3. 3. At a salinity of 20‰ rainbow trout on a restricted diet and held at 11°C have the highest growth rate. 4. 4. Owing to increasing RNA levels, the RNA/DNA quotient is significantly higher than normal in rainbow trout held at 1°C although the fishes do not grow at this temperature. 5. 5. Temperature and feeding both affect the enzymes we studied (liver: G1DH, AspT, arginase, G6PDH, and 6PGDH; kidney: G1DH, AspT, arginase, and Na/K-ATPase; white muscle: AspT and A1T; gill: Na/K-ATPase) differently. Interactions between these two factors also occur in some cases.
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