Abstract

The purpose of this experiment is to measure, by a sensitive manometric method, the rate of photosynthesis in a unicellular green alga as a function of light intensity at two temperatures under conditions where carbon dioxide is in abundant supply. A very large part of the research on photosynthesis has been done with cultures of unicellular green algae, which afford several conveniences compared to terrestrial seed plants. The unicellular algae can be grown in defined inorganic culture media under standard and reproducible conditions; uniform, dense, very active samples can be prepared and pipetted as a liquid suspension; and rate of photosynthesis can be accurately measured as rate of oxygen production by sensitive manometric methods.

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