Effects of temperature and dissolved oxygen on sperm energy metabolism and motility in the golden cuttlefish, Sepia esculenta

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 温度和溶解氧对金乌贼精子能量代谢和活性的影响 DOI: 作者: 作者单位: 1. 中国海洋大学, 海水养殖教育部重点实验室, 山东 青岛 266003;2. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266072 作者简介: 王展(1993-),男,硕士研究生,专业方向为渔业资源增殖学.E-mail:wangzhan@stu.ouc.edu.cn 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目（41676153）. Effects of temperature and dissolved oxygen on sperm energy metabolism and motility in the golden cuttlefish, Sepia esculenta Author: Affiliation: 1. Key Laboratory of Mariculture, Ministry of Education;Ocean University of China, Qingdao 266003, China;2. Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266072, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了解金乌贼（）精子对外界环境变化的响应和存活机制，分析测定了不同温度（16℃、20℃、24℃）和不同溶解氧（1 mg/L、3 mg/L、6 mg/L）条件下保存36 h后精荚能量物质含量、糖脂代谢关键酶活性以及精子激活后的活力变化。结果显示，温度和溶氧处理36 h后精荚糖原和甘油三酯含量均显著低于未处理组（>0.05）。当温度从16℃升高至24℃时，精荚糖原含量逐渐下降，甘油三酯的含量则先降后稳；己糖激酶（HK）、乳酸脱氢酶（LDH）、丙酮酸脱氢酶（PDH）和琥珀酸脱氢酶（SDH）活性随温度升高而升高，脂肪酶（LIP）活性先升后稳；精子激活初期运动速度和不运动率随温度升高无显著变化，而快速运动率则先稳后降。当海水溶解氧从1 mg/L升至6 mg/L时，精荚糖原含量逐渐下降，甘油三酯含量先稳后降；HK、PDH、SDH活性随溶解氧上升呈逐渐上升趋势，LDH先升后降，LIP则先稳后升；精子激活初期运动速度和快速运动率随溶解氧升高呈先升后稳的变化趋势，而不运动率则呈先降后稳趋势。上述结果表明，精荚中的糖原和甘油三酯是精子的主要供能物质，精子较低的能量物质消耗为其长期存活提供了保障，低温和低氧环境可抑制精子的耗能，更利于其在精荚中长期存活。 Abstract:In order to study the response and survival mechanism of spermatozoa in the external environment, the contents of energy substances, the activities of key glycolipid metabolizing enzymes in the spermatophore, and sperm motility after a 36-h preservation were examined under different temperatures (16℃, 20℃, 24℃) and dissolved oxygen levels (1 mg/L, 3 mg/L, 6 mg/L). The results showed that the glycogen and triglyceride contents in the spermatophore after the 36-h treatment were significantly lower than those before the 36-h treatment, but the protein content showed no significant differences before and after the 36-h treatment. When the water temperature increased from 16℃ to 24℃, the glycogen content decreased, while the triglyceride content first decreased and then stabilized. The activities of hexokinase (HK), lactate dehydrogenase (LDH), pyruvate dehydrogenase (PDH), and succinate dehydrogenase (SDH) increased when the water temperature increased, while the activity of lipase (LIP) first increased and then stabilized. The initial velocity and immotile rate after activation had no significant changes, but the progressive motility rate was first stable and then decreased when the water temperature increased. When the dissolved oxygen content increased from 1 mg/L to 6 mg/L, the glycogen content decreased, while the triglyceride content was first stable and then decreased. The activities of HK, PDH, and SDH increased, while the activity of LDH first increased and then decreased when the dissolved oxygen content increased. The activity of LIP was first stable and then increased, while the initial velocity and progressive motility rate after activation first increased and then stabilized, while the immotile rate first decreased and then stabilized. These results suggested that glycogen and triglycerides in the spermatophore were the main energy substances of the spermatozoa. Low energy substance consumption was the foundation for the long-term survival of the sperm. Low temperature and hypoxia, which reduced the energy consumption of the spermatozoa, were beneficial for its long-term survival. 参考文献 相似文献 引证文献