Abstract
Culture conditions were adapted to obtain larger biomass and higher docosahexaenoic acid(DHA)yield of Crypthecodinium cohnii under flask fermentation level.Results showed that the optimal culture should be at 22℃,in carbon-source culture with 12g/L glucose for 4d and then shifted to the same media but with 3g/L sodium acetate instead for 4d.Under this optimal condition,the C.cohnii biomass yield,DHA yield and DHA content reached 234.85mg/(L·d),17.45mg/(L·d),74.36mg/g dry weight respectively.
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