Effects of Taurine on Broiler Aortic Endothelial Cells Activity and Antioxidant Ability Impired by Heat Stress In Vitro.

Abstract

In order to provide a theoretical basis for the amelioration of heat stress-related diseases in broilers by taurine supplementation, the effect of taurine on the viability and antioxidant ability of aortic endothelial cells in broilers under heat stress was investigated in the present study. In this experiment, 10d healthy broilers were sacrificed, then aortic tissue was used for aortic endothelial cells isolation and cultivation. Tissue patching was used to cultivate primary broiler aortic endothelial cells. The 3rd to 5th generations of cells were used and randomly divided into five groups, including the control group (C), the heat-stressed group (HS), the Tau(HS+LTau) group, the Tau(HS+MTau) group and the Tau(HS+HTau) group. Cells were cultivated for 24h in a cell incubator (37°C, 5%CO2). Then heat-stressed cells were placed in a 43°C thermostatic water bath for 6h, followed by incubation in the cell incubator under 37°Cfor 1h. The results were as follows (1) Based on MTT colorimetry and AO/EB staining, the activity of aortic endothelial cells was decreased, but the rate of apoptosis was increased in the HS group. Compared with the HS group, the taurine groups showed significantly higher level in relative survival rates (P<0.05), and significantly lower apoptosis rates (P<0.05); (2) compared to control group, LDH activity and MDA content of endothelial cells in the HS group were significantly increased (P<0.01), while the levels of T-SOD, GSH-Px and T-AOC were significantly decreased (P<0.01). The LDH activity and MDA content of endothelial cells were significantly lower in Tau group than those of HS group (P<0.05), while the T-SOD activity, GSH-Px activity and T-AOC of endothelial cells were significantly increased (P<0.05) in the taurine group. The results show that HS decreases antioxidant capacity, which causes severe oxidative damage to the endothelial cells; while taurine administration prevents the decline in LDH activity and MDA content, and increases the activity of several antioxidant enzymes, including SOD, GSH-Px and T-AOC, which implies that taurine can improve the broiler aortic endothelial cells activity and antioxidant ability under heat stress.