Effects of tannins on Chinese hamster cell line B14

Abstract

Tannins, naturally occurring plant phenols, have been recognized as antioxidants, but toxic effects have also been observed. In the current investigation, the interaction of this type of compounds with Chinese hamster cells (cell line B14) has been examined. This study reports on the results of experiments in which B14 cells were exposed to tannins: tannic, ellagic and gallic acids in the concentration range 15–240 μM. The cytotoxic and genotoxic effects of these compounds were studied. The colorimetric MTT assay to assess cytotoxicity and the Comet assay for detection of DNA damage were used. In this paper, we also demonstrated the influence of tannins on the fluidity of the plasma membrane. This experiment was carried out by a spectrofluorometric method using two fluorescent probes: 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) and 12-(9-anthroyloxy)stearic acid (12-AS). The tannins increased the fluidity in the internal region of the lipid bilayer, but no changes at the surface of the plasma membrane were observed. The results of the MTT assay showed that tannins could decrease the viability of cells and that their cytotoxicity was highest at the concentration of 60 μM. The degree of toxicity of these compounds was not correlated with the concentration used. The data obtained from the Comet assay showed that the tannins could also contribute to formation of DNA single-strand breaks.