Effects of TanIIA on expressions of interleukin-1β and interleukin-6 in neonatal rats with periventricular leukomalacia

Abstract

Objective To study the effects of TanⅡA on expressions of interleukin-1β(IL-1β) and interleukin-6( IL-6) in brain tissues of neonatal rats with periventricular leukomalacia(PVL). Methods The PVL models were established by means of bilateral general cervical artery ligation followed by 0.5 h 80 mL/L oxygen and 920 mL/L nitrogen mixture exposure in 2-day rats, and then these rats were randomly divided into the model control group, the low-dose group treated with TaⅡA at 7.5 mg/(kg·d) and the high-dose group treated with TaⅡA at 15.0 mg/(kg·d), meanwhile the false operation group was established. The rats were killed 1 hour after administration on the third day, and the brain tissues pathological changes were detected by way of HE dyeing analysis. At the same time, the expressions of IL-1β and IL-6 in brain tissues were analyzed by means of enzyme linked immunosorbent assay(ELISA). Results HE staining showed that the cells around the lateral ventricle were disarranged, as white matter appeared sparse as cribriform and ischemic changed significantly in the rats of model control group. Compared with the model control group, the ischemia of brain tissue in the low-dose group improved. Ischemia of the high-dose TanⅡA group improved more significantly than the low-dose group. ELISA results showed that the expressions of IL-1β and IL-6 in the PVL rats were significantly higher than the false operation group (all P 0.05). Conclusions TanⅡA has the function of improving the state of ischemia and decreasing the expressions of IL-1β and IL-6 in tissues of PVL rats, thus it can relieve cerebral white matter lesions of neonatal rats. Key words: TanⅡA; Periventricular leukomalacia; Interleukin-1β; Interleukin-6; Neonatal rat