Effects of Taheri Consciousness Fields on the Rat C6 Glioma Cell Line

Abstract

The most common primary spinal cord and brain tumors are gliomas, representing 81% of malignant brain tumors. In neuro-oncology investigations, the rat C6 glioma cell line has mostly been utilized as an experimental model system. Taheri Consciousness Fields (TCFs) founded and introduced by Mohammad Ali Taheri, are novel fields that are neither matter nor energy. Therefore, they are non-quantifiable and cannot be directly observed or measured. However, it is possible to demonstrate and measure the effects of these fields through standard scientific experiments. This study aimed to evaluate the effect of TCFs (A and B) on the C6 glioma cell line in three and four treatments compared with the control (nono-treatment) group. To study morphology and microscopic properties of the cells, cells were detached and stained with trypan blue then the dead cells were counted. An MTT assay was used in order to evaluate the effect of TCFs. The expression of Bax and Bcl-2 genes was assessed by using the RT Real-time PCR. The results showed that the TCFs treatment not only reduced the number of the C6 glioma cells but also changed their morphology. Similarly, in the trypan blue dye count, more dead cells were counted in the TCFs groups in comparison to the control group. In the MTT assay, both TCFs displayed a cytotoxic effect in incubation times on the C6 glioma cells (p< 0.05). Bax/Bcl2 ratio increased to 3.5 and 7-fold, compared to the control via TCF (A), and (B), respectively (P<0.05). The findings suggest that TCF (A) and (B) can induce apoptosis in the rat C6 glioma cells. The TCF (B) effect was greater than the effect of TCF (A) in all tests. The mechanism of action of TCFs is still not definable by researchers, and it can be useful to elucidate the effects of the TCFs treatment in vivo and in clinical research.