Abstract
Syringin is a phenylpropanoid glycoside isolated from the bark of Phellodendron chinensis Schneid. The present work was designed to examine the effects of syringin on the protection and expression levels of adhesion molecules in human umbilical vein endothelial cells (HUVECs) induced by monosodium urate (MSU). Syringin treatment increased the MSU-induced HUVEC viability as assessed by the 3-(4, 5-dimeth-ylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Syringin also suppressed the expression levels of MSU-induced endothelial cell intercellular adhesion molecule-1 as assessed by reverse transcription polymerase chain reaction (PCR). Syringin treatment decreased MSU-induced HUVEC apoptosis as well, as assessed by acridine orange/ethidium bromide staining and flow cytometry analysis. Overall, the present study suggests that syringin suppresses the expression levels of MSU-induced inflammation and is effective against acute gout. Key words: Syringin, acute gout, human umbilical vein endothelial cells, cell viability, apoptosis, intercellular adhesion molecule-1(ICAM-1).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.