Effects of supplementation of coated crystalline amino acids on growth performance and body composition of juvenile kuruma shrimp Marsupenaeus japonicus

Abstract

Supplementation of crystalline amino acids (CAA) in shrimp diets is unsuccessful in most cases. In the present study, various pre-coated CAA were developed to minimize the leaching losses from diets in water and assessed using kuruma shrimp Marsupenaeus japonicus juveniles. Experimental diets were supplemented with coated CAA or non-coated CAA to simulate the dietary amino acid profile to that found in the whole body protein of shrimp. The four diets contained CAA coated with either carboxymethyl cellulose (CMC) (diet 1), zein (diet 2), k-carrageenan (diet 3) or agar (diet 4), respectively. Another four diets contained CMC-coated CAA with the addition of either zein (diet 5), casein-gelatin (diet 6), zein and k-carrageenan (diet 7), or casein-gelatin and k-carrageenan (diet 8), respectively. The 8 weeks feeding trial demonstrated that the diets containing coated CAA gave significantly higher weight gains than diets containing non-coated CAA, with the highest value for the diet 6. The diets containing coated CAA also yielded higher feed conversion efficiency, protein efficiency ratio, and specific growth rate than diets containing non-coated CAA. Leaching trials suggested that shrimp growth performance was improved partly due to the retardation or reduction of CAA leaching by coating them with specific binders.