Abstract
To analyse the effects of supplemental autologous seminal plasma on boar semen quality before freezing and after thawing, thirty ejaculates were collected from six Pietrain boars. The main factors of a 2 x 2 x 2 factorial arrangement of treatments were Beltsville thawing solution (BTS), seminal plasma before freezing, and seminal plasma after freezing-thawing. The percentage of acrosome-intact sperms was reduced by semen dilution. There were no interactions of main factors. The addition of seminal plasma to semen before freezing did not affect semen quality, but the addition of seminal plasma after freezing-thawing increased the percentage of acrosome-intact sperm. This approach indicates that the addition of seminal plasma to boar semen after freezing-thawing improves semen quality. Keywords: Acrosome integrity, freezing, thawing, semen
Highlights
Freeze-thawed boar semen has limited commercial use because during the freezing-thawing process, 50% of the spermatozoa lose their fertilizing capacity (Qian et al, 2016) as a result of thermal shock, which compromises viability and integrity of DNA and increased lipid peroxidation of membrane phospholipids (Fraser et al, 2017)
Seminal plasma was obtained from the same boars at the time that the semen samples were collected and processed according to the strategies of boar sperm cryopreservation of Okazaki & Shimada (2012)
Raw fresh semen had 85% viability, but thawed semen after freezing had 60% viability. This means that the freezing-thawing process reduced almost 24% of semen viability
Summary
Freeze-thawed boar semen has limited commercial use because during the freezing-thawing process, 50% of the spermatozoa lose their fertilizing capacity (Qian et al, 2016) as a result of thermal shock, which compromises viability and integrity of DNA and increased lipid peroxidation of membrane phospholipids (Fraser et al, 2017). The sperm-rich portions of the ejaculate were evaluated for conventional semen characteristics (volume, sperm concentration and percentage of motile spermatozoa) using standard laboratory techniques (Gutiérrez-Pérez et al, 2009). Half (50 mL) of the total semen sample was extended (1 : 1) with Beltsville thawing solution (BTS) (Sigma-Aldrich Co., St. Louis, MO, USA)
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