Abstract
Several methods were used to test the hypothesis that α‐amylase activity in pea stem tissues is regulated by catabolite repression. The presence of various exogenous sucrose concentrations had little effect upon the accumulation and subsequent loss of enzyme activity in excised embryonic axes. Comparable results were observed for glucose, fructose, and maltose, and mixtures of sucrose and glucose. While growth and sugar accumulation was increased by culturing the axes in liquid medium, the activity was not reduced. Therefore the results of each procedure were inconsistent with the initial hypothesis. Preliminary experiments also demonstrated the lack of influence of sugars upon the in vitro activity of pea α‐amylase.
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