Abstract
The effects of substrate composition and high volumetric loading rate on the characteristics of substrate removal and biofilm formation in methanogenic phase of anaerobic two-phase digestion were investigated by using laboratory-scale anaerobic fluidized reactors. First, the effects of substrate composition were studied using three kinds of substrate. The first one was composed of acetic acid, and second and third ones were composed of acetic acid, propionic acid and butyric acid. A small amount of yeast extract and glucose were added to the second and third ones, respectively. In the experiments in which yeast extract or glucose was added to the mixed acid substrate, the substrate removal efficiencies were higher than those in the experiment using acetic acid substrate. The biofilm formation was accelerated by using the mixed substrate to which yeast extract was added. Secondly, the effects of high volumetric loading rate were studied using acetic acid substrate and mixed acid-glucose substrate, respectively. In both cases, substrate removal efficiencies of the reactors were remarkably high at a loading rate of 30kg COD·m−3·d−1. However, the effluent quality of that reactor to which acetic acid was added was unstable.
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