Abstract
The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes.
Highlights
Cell migration is directed by various chemical and physical cues, phenomena named “-taxis”.Chemotaxis, the directional migration of somatic cells in response to certain chemical gradient, plays important roles in many physiological processes such as cancer metastasis [1,2] and cell development [3,4]
Electrotaxis, characterizing how adherent cells move under the stimulus of direct current or alternating current electric fields, has been reported to be crucial in wound healing [5,6]
To get rid of the drawbacks of the scratching method, here we report a modified barrier wound-healing assay for investigating collective cell migration on different coated substrates
Summary
Cell migration is directed by various chemical and physical cues, phenomena named “-taxis”. Chemotaxis, the directional migration of somatic cells in response to certain chemical gradient, plays important roles in many physiological processes such as cancer metastasis [1,2] and cell development [3,4]. Electrotaxis, characterizing how adherent cells move under the stimulus of direct current or alternating current electric fields, has been reported to be crucial in wound healing [5,6]. To follow the dynamic process of collective cell migration as well as better understand its underlying mechanisms, researchers have developed various in vitro techniques, including wound-healing assays [13,14]
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