Abstract
Safer alternatives to synthetic pesticides are essential for sustainable agriculture. Methyl benzoate (MB) is a volatile essential oil found in several plants. Recent reports of the toxicity of MB to arthropod pests suggest that MB may be a useful alternative insecticide. The present study assessed the effects of a sublethal concentration of MB (LC30, 0.22%) on the life history and reproductive characteristics of the cotton aphid, Aphis gossypii Glover, in both a treated parental generation (F0) and untreated progeny (F1). MB treatment significantly decreased longevity and fecundity in both the F0 and F1 generations, and prolonged the developmental duration of each immature instar of the F1 generations, compared with controls. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) of the F1 generation were significantly reduced, compared to controls. The mode of action of MB is not known, but in aphids treated with LC30 MB, the activity of the enzyme acetylcholinesterase (AChE) decreased by more than 65%, compared with untreated controls. AChE activity was rapidly inhibited within 1 h, and remained inhibited for 6 h after in vivo exposure to MB. Moreover, molecular docking analysis revealed that MB had a strong affinity with the catalytic site of AChE, with a binding energy value of −6.2 kcal/mole. Our results suggest that MB targets AChE, and that a sublethal dose of MB can have adverse transgenerational effects on cotton aphids.
Highlights
The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a common agricultural insect pest that attacks crops worldwide [1]
We evaluate the effects of a sublethal concentration of methyl benzoate (MB) on selected biological parameters of cotton aphids
The colony was established on cucumber plants (Cucumis sativus L.) in a laboratory maintained at 25 ± 1 ◦ C and 60 ± 10% relative humidity (RH), with a 16:8 h light/dark photoperiod
Summary
The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a common agricultural insect pest that attacks crops worldwide [1]. The cotton aphid damages plants directly by consuming. Agronomy 2020, 10, 1313 sap, and indirectly by transmitting viral diseases and contaminating plants with honeydew [2,3]. Cotton aphids are carriers of at least 76 viral diseases, threatening 900 known host plants [1]. Synthetic chemical insecticides are the most common current control methods for cotton aphids [4,5]. Cotton aphids have developed resistance to many pesticides, due to frequent use [6,7,8]. The side effects of synthetic pesticide use include environmental hazards, residue problems, and negative impacts on natural enemies, reiterating the importance of finding alternative control methods
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