Effects of Subcutaneous Microcrystalline Cellulose Sulfate Extracted from the Wood of the Siberian Fir (Abies sibirica Ledeb) on the Clotting of Rabbit Plasma

Abstract

The anticoagulant activity of the microcrystalline cellulose sulfate (MCCS) extracted from the wood of Abies sibirica Ledeb (molecular weight 86,000, sulfur substitution level 1.63) were studied in vitro and ex vivo. The antithrombin activity was 87.4 ± 0.7 U/mg and activity against activated factor X was 18.3 ± 0.2 U/mg. S.c. administration of high doses (5, 20, and 40 mg/kg) of MCCS to rabbits increased plasma antithrombin activity. A cellulose sulfate dose of 10 mg/kg produced the same area under the pharmacodynamic curve for plasma antithrombin activity as unfractionated heparin (5 mg/kg).