Effects of sub-chronic aluminum chloride on spermatogenesis and testicular enzymatic activity in male rats

Abstract

AimsThe aim of this study was to determine the effects of sub-chronic aluminum chloride (AlCl3) on spermatogenesis and testicular enzymatic activity in male rats. Main methodsForty Wistar male rats were randomly divided into four groups: control group (CG, 0), low-dose group (LG, 64.18mg/kg BW AlCl3), mid-dose group (MG, 128.36mg/kg BW AlCl3) and high-dose group (HG, 256.72mg/kg BW AlCl3). The rats were orally administered with AlCl3 for 120days. At the end of the experiment, the contents of Al, Fe, Cu and Zn, the enzyme activities of testicular acid phosphatase (ACP), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), lactate dehydrogenase isoenzyme (LDH-x), the sperm count and the sperm malformation rate were examined. Key findingsThe results showed that the Al and Cu contents, sperm count and the enzyme activities of testicular ACP, SDH, LDH and LDH-x decreased, while the Zn and Fe contents and sperm malformation rate increased in AlCl3-treated rats. SignificanceIt suggests that sub-chronic AlCl3 disorders the balance of trace element and decreases the spermatogenesis and the activities of testicular enzymes, indicating that AlCl3 has adverse effect on the testicular function in male rats.