Effects of structure and composition of decellularized liver biological scaffolds on cytocompatibility

Abstract

Objective To compare the biocompatibility of decellularized liver biological scaffolds (DLBS) made by different methods and discussing the correlation between them.Methods Decellularized liver biological scaffolds were made by transfusing livers of 50 mature F344 rats with three different ways.Glycosaminoglycan (GAG) in the scaffolds was tested,and scanning electron microscope (SCM) and cytotoxicity assays were also used on the scaffolds.The liver oval cells were transfused into scaffolds through the portal vein.Cells on the scaffolds were examined by immunofluorescence (IF),scanning electron microscope,cells adhesion test and albumin excretion detection.Results Scaffolds made by the Method C had better orderliness than other two methods in gross observation,scanning electron microscopy and pore size calculation.The Method C [(44.19 ±3.35) ng/mg] had significantly more GAG than the Method A [(21.63 ±2.78) ng/mg] and Method B [(36.71 ±2.01) ng/mg].The scaffolds made by the Method C [(95.78 ± 2.11) ％] had higher collagen than in those made by the Method B [(91.15 ± 2.21) ％] and Method A [(84.18 ± 3.30)％].The cell adhesion rate of the Method B and Method C was higher than that of the Method A.Albumin excretion in the Method C [(85.77 ± 3.30) mg/106 cells] was greater than in the Method A [(49.37 ±2.43) mg/106 cells] and Method B [(74.66 ±4.80) mg/106 cells].Conclusion DLBS can be prepared by all three ways,but scaffolds made by our new method have better biocompatibility,and our method has significant advantages over the other methods. Key words: Decellularized liver scaffolds ; Tissue engineering; Extracellular matrix; Hepatic oval cell