Abstract
Metabolomic studies were carried out using gas chromatography and mass spectrometry (GC‐MS) on Daohuaxiang variety rice (Oryza sativa L.) from the Wuchang Geographical Indication Rice Protection Area in Heilongjiang Province, to investigate the effects of storage on brown rice metabolism. The data were subjected to principal component analysis (PCA), orthogonal partial least squares‐discriminant analysis (OPLS‐DA), and cluster analysis using software such as SIMCA. Analysis of the samples led to the identification of a total of 160 metabolites. No significant differences were found in the amount of metabolites before and after storage. A total of 31 differential metabolites were screened, and the changes in metabolite content showed a “reverse change” overall. Storage significantly changed the content of various metabolites in rice, with fatty acids impacted most significantly. Metabolic pathway analysis revealed that fatty acid biosynthesis is a key metabolic pathway in rice storage. The degradation of brown rice quality caused by storage is closely related to the composition and content of its metabolites, and that change in lipid content significantly affects brown rice quality during storage.
Highlights
Metabolomics is the study of the endogenous metabolites in organisms and their changes
Based on the metabolomics technology of gas chromatography and mass spectrometry (GC-MS), a metabolomics analysis was performed on stored brown rice
The results showed that storage had a small effect on the amount of brown rice metabolites and a large effect on the composition of metabolites
Summary
Metabolomics is the study of the endogenous metabolites in organisms and their changes It is a science utilizing qualitative and quantitative analysis of all low molecular weight (
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