Abstract
Platelet-free plasma of human blood (sodium citrate and EDTA as an anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at 37°C for 24 hours. RBC aggregation decreased after incubation of plasma and serum at 37°C for 4 hours. The RBC shape was changed at the same time: discocytes transformed to echinocytes. Storage of plasma and serum at 4°C and room temperature did not lead to significant alterations of RBC aggregation. The RBC shape did not change in influence of such plasma and serum. The most considerable decrease of RBC aggregation and change of their shapes were observed in the plasma and serum incubated at 37°C for 24 hours. Dilution of incubated plasma by fresh plasma led to consistent restoration of erythrocyte shape and their aggregation.
Highlights
Human red blood cell (RBC) aggregation is determined by two types of biophysical and physicochemical factors: suspending phase properties and RBC properties [1,2]
We investigated the influence of human autologous plasma and serum after being stored for 24 hours at 4 ̊C, room temperature (25 ̊C) and at 37 ̊C, on the RBC aggregation and shape
The aggregation of washed erythrocytes in fresh autologous plasma anticoagulated with sodium citrate did not differ from erythrocyte aggregation in fresh plasma anticoagulated with EDTA
Summary
Human red blood cell (RBC) aggregation is determined by two types of biophysical and physicochemical factors: suspending phase properties and RBC properties [1,2]. Plasma proteins, such as fibrinogen, α2-macroglobulin, and immunoglobulins play a crucial role in the RBC aggregation [3,4,5]. We investigated the influence of human autologous plasma and serum after being stored for 24 hours at 4 ̊C, room temperature (25 ̊C) and at 37 ̊C, on the RBC aggregation and shape
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