Effects of storage duration and storage media on initial and post-eyeing mortality of stored ova of rainbow trout Oncorhynchus mykiss

Abstract

The effects of storage duration and storage media on the viability of unfertilized eggs of rainbow trout from fertilization to hatching stage was studied. The unfertilized eggs were stored in coelomic fluid and Cortland artificial media buffered with Tris–HCl (C 4H 11NO 3–HCl) and Hepes (C 8H 18N 2O 4S) at 2–3 °C. These eggs were fertilized after 0 (i.e. control eggs fertilized prior to storage), 2 and 9 days post-stripping using fresh, pooled sperm obtained from 4 to 5 males. According to the results of present study, after 2 days of storage, no significant ( p > 0.05) change in survival to eyeing and hatching of eggs stored in coelomic fluid (85.5 ± 4.8%, 80.2 ± 6.3%) and Cortland medium buffered with Hepes (73.3 ± 4.1%,68.1 ± 4%) was observed in comparison to the control (82.6 ± 8%,78.7 ± 7.8%). However, eyeing and hatching survival rates of eggs stored for the same period in Cortland medium buffered with Tris–HCl (44.5 ± 7%,34.2 ± 8.2%) decreased significantly ( p < 0.05) in comparison with the control. Also, eggs stored for 2 days in Cortland medium buffered with Tris–HCl (25 ± 6.7%) had significantly ( p < 0.05) greater eyed egg mortality compared to the control (4.7 ± 0.6%), while no significant ( p > 0.05) differences were observed between eyed egg mortality rates of eggs stored in coelomic fluid (6.7 ± 2.1%) and Cortland medium buffered with Hepes (7.3 ± 0.9%) compared to control. After 9 days of storage, the eggs that were stored in coelomic fluid showed no significant ( p > 0.05) differences in eyeing (77.5 ± 7.2%) and hatching (67 ± 8.1%) rates in comparison with control, while both Cortland storage media exhibited significant ( p < 0.05) loss in eyeing (44.4 ± 11.5%, 24.7 ± 13.8% for Hepes and Tris–HCl, respectively), and hatching (24.5 ± 5.9%, 19.2 ± 14.4% for Hepes and Tris–HCl, respectively) in comparison with control. Furthermore, eyed egg mortality increased significantly ( p < 0.05) in coelomic fluid (14 ± 2.9%), media buffered with Hepes (43 ± 6.2%) and Tris–HCl (49.3 ± 26.9%) compared to control. Based on this study, unfertilized eggs of rainbow trout can be successfully stored in coelomic fluid for 9 days at (2–3 °C) without significant loss of fertility. However, storage within a similar period in artificial media showed lower fertilization rates and higher eyed egg mortality compared to coelomic fluid.