Effects of starvation on respiratory metabolism and energy metabolism in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

Abstract

Intermittent food shortages are commonly encountered in the wild. To cope with the threat of starvation, insects initiate a suite of behavioral activities and physiological countermeasures. The cotton bollworm, Helicoverpa armigera, is a major agricultural pest worldwide, but how H. armigera modulates its metabolism under starvation remains ambiguous. In the present study, the respiratory rates (V̇O2; ml g−1 h−1) from the third-larval instar to the pupal stage were first determined. Our results highlighted a transient rise during the larval-larval molt and larval-pupal transition, followed by a sharp decline in the pupal stage and, finally, an upward trend before eclosion. When subjected to food deprivation, the starved larvae experienced a significant decline in the rates of O2 consumed and CO2 produced, as well as in respiratory quotient (RQ) values, indicative of severe metabolic depression during starvation and a shift of metabolic substrates with prolonged starvation. For metabolic substrate analysis, an apparent decline in triglyceride and glycogen contents was observed in the starved larvae, and the hemolymph trehalose content was significantly reduced throughout starvation. In addition, comparative transcriptome analysis showed that 48 h of larval starvation caused substantial transcriptional regulations in several energetically costly processes, wherein the marked up-regulations were detected in the pathways of glycolysis and fatty acid metabolism. Overall, our work makes a comprehensive study on the respiratory rate and energy metabolism in the starved H. armigera larvae, and provides a deep insight into the physiological adaptive strategies to alleviate nutritional stress.