Abstract
Effects of standardized Wrightia tinctoria seeds extract (WTSE) and Stereospermum suaveolens root extract (SSRE) on erythrocyte membrane stabilization, mast cell protection, nitric oxide, and pro-inflammatory cytokines production were evaluated by in vitro methods. WTSE and SSRE were standardized against chlorogenic acid and lapachol, respectively, using reverse-phase high-performance liquid chromatography. Standardized WTSE and SSRE inhibited erythrocyte membrane lysis, mast cell degranulation, and LPS-stimulated nitric oxide and pro-inflammatory cytokine (IL-β, IL-6 and TNF-α) production by RAW 264.7 macrophage cells in a dose-dependent manner.
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