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Abstract
ABSTRACT This study was conducted to compare the quantity of total protein in miniature pig sperm extract (SE) extracted by two methods [sonication and a method using Mammalian Protein Extraction Reagent (M-PER)]. Furthermore, the effects of injection with SE extracted by M-PER and fractionated SE on porcine oocyte activation were examined. The total amount of protein extracted from miniature pig spermatozoa (1 ×109 sperm) by M-PER was significantly (P 100 kDa and 100 kDa, and <100 kDa groups were 63.6, 48.7, and 46.4%, respectively. Significa...
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