Effects of specific immunotherapy on the expression levels of serum IL-17,IL-35 and Treg/Th17 regulatory T cellsin patients with allergic rhinitis caused by dermatophagoides

Abstract

Objective:To explore the regulatory effect of sublingual immunotherapy on the balance of Treg/Th17 cells and the expression of IL-17 and IL-35 in serum of allergic rhinitis(AR) in pre-specific and post-specific immunotherapy.Method:In this study,30 cases were randomly selected from outpatients of otolaryngological department in the second hospital of Hebei Medical university.These were attributed as pretherapy group.After treatment,the same patients were as renamed as the post-therapy group.Another 30 cases were healthy subjects enrolled from physical examination branch of our hospital.We detected the expression level of IL-35 and IL-17 in peripheral blood by using ELISA and defeced CD4⁺ CD25⁺ Foxp3⁺ T cell and CD4⁺ IL-17⁺ T cell expression level via flow cytometry.Result:The expression level of IL-17 in pre therapy group was obviously higher than that in control group(P<0.05);The expression level of IL-17 in post therapy group was obviously lower than that in pre-therapy group,The difference was a statistically significance(t=5.030,P<0.05);The expression level of IL-17 in post therapy group was also higher than that in control group(P <0.05 ).The expression level of IL-35 in pre-therapy group was obviously lower than that in control group(P<0.05);The expression level of IL-35 in post therapy was obviously higher than that in pre-therapy group;The difference was a statistically significance (t=-4.083,P<0.05),the expression level of IL-35 in post therapy group was also lower than that in control group(P<0.05).The percentage of CD4⁺CD25⁺Foxp3⁺ T cell in CD4⁺ T cell was significant lower in pre therapy group than that in control group (P<0.05);The percentage of CD4⁺CD25⁺Foxp3⁺ T cell in CD4+ T cell in post therapy was obviously higher than that in pre therapy group;The difference was a statistically significance(t=-10.584,P<0.05),The percentage of CD4⁺CD25⁺Foxp3⁺ T cell in CD4⁺ T cell was also lower in post therapy group than that in control group (P<0.05 ).The percentage of CD4⁺IL17⁺ T cell in CD4⁺ T cell was significant higher in pre therapy group than that in control group (P<0.05);The percentage of CD4⁺IL-17⁺ T cell in CD4⁺ T cell in post therapy group was obviously lower than that in pre therapy group.The difference was a statistically significance (t=6.258,P<0.05).The percentage of CD4⁺IL-17⁺ T cell in CD4⁺ T cell was also higher in post therapy group than that in control group (P<0.05 ).Conclusion:Specific immunotherapy can have an impact on the expression levels of IL-17,IL-35 and also on Treg/Th17 cells balance in peripheral blood for patients with allergic rhinitis.