Effects of Spatial Variability and Drainage on Extracellular Enzyme Activity in Coastal Freshwater Forested Wetlands of Eastern North Carolina, USA

Abstract

Drainage of freshwater wetlands is common in coastal regions, although the effects on microbial extracellular enzyme activity (a key mediator of soil organic matter decomposition) in relation to spatial variability (microtopography and soil depth) are poorly understood. Soils were collected from organic (Oi, Oe, Oa) and mineral (A, AB, B) horizons from a natural and drained coastal forested wetland in North Carolina, USA. Activity of seven enzymes were measured: α-glucosidase (AG), β-glucosidase (BG), cellobiohydrolase (CBH), xylosidase (XYL), phenol oxidase (POX), peroxidase (PER) and N-acetyl glucosamide (NAG). Enzyme activity rates were normalized by soil weight, soil organic C (SOC), and microbial biomass C (MBC). Specific enzyme activity (per SOC or MBC) was more sensitive to drainage and soil depth compared to normalization by soil weight. In Oi and Oa horizons, specific enzyme activity (per MBC) (AG, BG, XYL, POX, PER) was higher in the natural compared to drained wetland but lower (AG, CBH, XYL, POX, PER, NAG) in the AB or B mineral soils. Results from this study indicate that organic soil horizons of natural freshwater wetlands contain a highly active microbial community driven by inputs of plant-derived C, while deeper soils of the drained wetland exhibit higher microbial metabolic activity, which likely plays a role in SOC storage of these systems.