Abstract
This study was developed to provide further information on the intestinal barrier permeability and the tight junction protein expression in weaned piglets fed with different levels of soybean agglutinin (SBA). Twenty-five weaned crossbred barrows (Duroc × Landrace × Yorkshire) were selected and randomly allotted to five groups, each group with five replicates. The piglets in the control group were not fed with leguminous products. 0.05, 0.1, 0.15 and 0.2% SBA was added to the control diet to form four experimental diets, respectively. After the experimental period of 7 days (for each group), all the piglets were anesthetized with excess procaine and slaughtered. The d-lactic acid in plasma and the Ileal mucosa diamine oxidase (DAO) was analyzed to observe the change in the intestinal permeability. The tight junction proteins occludin and ZO-1 in the jejunum tissue distribution and relative expression were detected by immunohistochemistry and Western Blot. The results illustrated that a high dose of SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no significant affects. The contents of DAO, d-lactic acid, occludin or ZO-1, had a linear relationship with the SBA levels (0–0.2%) in diets. The high dose SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no affects.
Highlights
Lectins, a kind of glycoprotein compound, can bind to receptors of intestinal epithelial cells [1].Further effects of lectins include changing the gut immune function, reducing production of endocrine cells and gut hormones, disordering the bacterial balance in the gut lumen, and damaging mucosal cells [2]
The piglets in the control group were fed without ingredients originating from leguminous products. 0.05, 0.1, 0.15 and 0.2% Soybean agglutinin (SBA) was added to the control diet in order to form four experimental diets, respectively
Ileal mucosa diamine oxidase (DAO) in piglets fed 0.1, 0.15 and 0.2% SBA decreased compared to the control and
Summary
A kind of glycoprotein compound, can bind to receptors of intestinal epithelial cells [1]. Soybean agglutinin (SBA), being one of the major ANFs, is resistant to digestive enzymes in the gastrointestinal tract, which has a unique property of binding to carbohydrate-containing molecules. Weaning increases the intestinal barrier permeability, and decreases the tight junction protein expression contents of occludin and ZO-1 for piglets [12,13]. The effects of SBA on intestinal barrier permeability and function are not yet fully elucidated to our knowledge. This study was developed to provide further information on the intestinal barrier permeability and the tight junction protein expression in weaned piglets fed with different levels of SBA, which are essential criteria to explore the mechanism of SBA-injured intestinal barrier function
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